Corticostriatal Neurons in the Anterior Auditory Field Regulate Frequency Discrimination Behavior / 神经科学通报·英文版

The anterior auditory field (AAF) is a core region of the auditory cortex and plays a vital role in discrimination tasks. However, the role of the AAF corticostriatal neurons in frequency discrimination remains unclear. Here, we used c-Fos staining, fiber photometry recording, and pharmacogenetic manipulation to investigate the function of the AAF corticostriatal neurons in a frequency discrimination task. c-Fos staining and fiber photometry recording revealed that the activity of AAF pyramidal neurons was significantly elevated during the frequency discrimination task. Pharmacogenetic inhibition of AAF pyramidal neurons significantly impaired frequency discrimination. In addition, histological results revealed that AAF pyramidal neurons send strong projections to the striatum. Moreover, pharmacogenetic suppression of the striatal projections from pyramidal neurons in the AAF significantly disrupted the frequency discrimination. Collectively, our findings show that AAF pyramidal neurons, particularly the AAF-striatum projections, play a crucial role in frequency discrimination behavior.

Functional Autapses Form in Striatal Parvalbumin Interneurons but not Medium Spiny Projection Neurons / 神经科学通报·英文版

Autapses selectively form in specific cell types in many brain regions. Previous studies have also found putative autapses in principal spiny projection neurons (SPNs) in the striatum. However, it remains unclear whether these neurons indeed form physiologically functional autapses. We applied whole-cell recording in striatal slices and identified autaptic cells by the occurrence of prolonged asynchronous release (AR) of neurotransmitters after bursts of high-frequency action potentials (APs). Surprisingly, we found no autaptic AR in SPNs, even in the presence of Sr2+. However, robust autaptic AR was recorded in parvalbumin (PV)-expressing neurons. The autaptic responses were mediated by GABAA receptors and their strength was dependent on AP frequency and number. Further computer simulations suggest that autapses regulate spiking activity in PV cells by providing self-inhibition and thus shape network oscillations. Together, our results indicate that PV neurons, but not SPNs, form functional autapses, which may play important roles in striatal functions.

Cancer incidence estimates for 2022 & projection for 2025: Result from National Cancer Registry Programme, India

Background & objectives: Information on recent cancer statistics is important for planning, monitoring and evaluating cancer control activities. This article aims to provide an update on the cancer incidence estimates in India by sex, age groups and anatomical sites for the year 2022. Methods: The National Cancer Registry Programme Report 2020, reported the cancer incidence from 28 Population-Based Cancer Registries (PBCRs) for the years 2012-2016. This was used as the basis to calculate cancer estimates in India. Information pertaining to the population at risk was extracted from the Census of India (2001 and 2011) for the estimation of age–sex stratified population. PBCRs were categorised into the respective State and regions of the country to understand the epidemiology of cancer. The age-specific incidence rate for each specific anatomical site of cancer was applied to the estimated population to derive the number of cancer cases in India for 2022. Results: The estimated number of incident cases of cancer in India for the year 2022 was found to be 14,61,427 (crude rate:100.4 per 100,000). In India, one in nine people are likely to develop cancer in his/her lifetime. Lung and breast cancers were the leading sites of cancer in males and females, respectively. Among the childhood (0-14 yr) cancers, lymphoid leukaemia (boys: 29.2% and girls: 24.2%) was the leading site. The incidence of cancer cases is estimated to increase by 12.8 per cent in 2025 as compared to 2020. Interpretation & conclusions: The cancer incidence is continuing to increase in India. The new estimates will be helpful in planning cancer prevention and control activities through the intervention of early detection, risk reduction and management.

Proyección de brecha de especialistas en nefrología en Uruguay 2020-2050: aplicación de un modelo de dinámica de sistemas

Objetivo Estimar el equilibrio/desequilibrio entre la oferta y la necesidad de nefrólogos en Uruguay para el año 2020 así como la tendencia hacia el 2050. Métodos Se desarrolló un modelo de simulación, aplicando la metodología de dinámica de sistemas con dos componentes: el de oferta y el de necesidad. Se definió 2020 como el año base y un horizonte de proyección hasta 2050. Se comparó la oferta y la necesidad en el periodo de proyección para establecer la brecha tanto en términos absolutos como relativos. Resultados La proyección de brecha para todos los escenarios considerados es de superávit en la mayor parte del periodo analizado. Conclusiones El trabajo aporta insumos respecto de aquellos parámetros sobre los que se puede incidir y que afectan a la oferta de especialistas. El valor de las proyecciones radica en su utilidad para identificar la situación actual y las tendencias futuras a las que deben responder los responsables de la formulación de políticas.

Objective To estimate the balance/imbalance between supply and demand of nefrologists in Uruguay for the year 2020, adding its tendency towards 2050. Methods A simulation model was developed, applying a two dynamic systems methodology with two components: that of supply and that of need. The base year was defined as 2020 with a projection horizon up to 2050. Supply and need were compared for the projection period to establish the gap, both in relative and absolute terms. Results The projection gap for all considered scenarios is that of surplus for most of the analyzed period. Conclusions This work offers input with respect of impactful parameters that affect healthcare specialists supply. The key value of the projections lies in its utility to identify the current situation and its future tendencies, to which those responsible for policy making have to respond.

Value of dual energy CT parameters combined with serum procollagen Ⅰ N-terminal propeptide and beta C-terminal cross-linked telopeptide of type Ⅰ collagen in differential diagnosis of spinal bone metastasis from lung cancer and myeloma / 中国医师进修杂志

Objective:To analyze the value of dual energy CT parameters combined with serum procollagen Ⅰ N-terminal propeptide (PⅠNP) and beta C-terminal cross-linked telopeptide of type Ⅰ collagen (β-CTX) in differential diagnosis of spinal bone metastasis from lung cancer and myeloma.Methods:The clinical data of 54 patients with spinal bone metastasis from lung cancer and 50 patients with myeloma in Jincheng People′s Hospital from October 2019 to March 2021 were analyzed retrospectively. All patients were examined by dual energy CT on the day of admission, and the CT values at the energy levels of 40 to 80 keV (energy interval of 10 keV) were recorded. The serum PⅠNP and β-CTX levels were detected by chemiluminescent assay before treatment. The pathological examination results were taken as gold standard, and the CT values at the energy levels of 40 to 80 keV by dual energy CT and serum PⅠNP and β-CTX levels were compared between 2 groups. Receiver operating characteristic (ROC) curve was used to analyze the differential diagnosis value of the CT values at the energy levels of 40 to 80 keV, serum PⅠNP and β-CTX levels alone and combination.Results:The CT values at the energy levels of 40 to 80 keV by dual energy CT and serum PⅠNP and β-CTX levels in patients with spinal bone metastasis from lung cancer were significantly higher than those in patients with myeloma: 79.86 (61.20, 116.32) HU vs. 58.29 (46.92, 64.03) HU, 64.48 (50.27, 90.08) HU vs. 45.78 (38.59, 56.75) HU, 57.35 (43.31, 78.04) HU vs. 43.62 (36.91, 54.06) HU, 52.05 (42.98, 75.79) HU vs. 41.26 (32.84, 51.76) HU, 45.52 (38.55, 63.59) HU vs. 36.68 (28.72, 49.83) HU, 66.35 (31.15, 81.97) μg/L vs. 31.38 (27.76, 34.50) μg/L and 0.61 (0.48, 0.67) μg/L vs. 0.49 (0.47, 0.52) μg/L, and there were statistical differences ( P<0.05 or <0.01). ROC curve analysis result showed that the sensitivity of the combination of the CT values at the energy levels of 40 to 80 keV by dual energy CT was higher than those alone (83.33% vs. 59.26%, 61.11%, 62.96%, 64.81% and 66.67), the area under the curve (AUC) was also higher than those alone (0.882 vs. 0.798, 0.811, 0.817, 0.801 and 0.773), and there were statistical differences ( P<0.01 or <0.05); the sensitivity of the combination of serum PⅠNP and β-CTX levels was higher than those alone (81.48% vs. 57.41% and 62.96%), the AUC was higher than those alone (0.829 vs. 0.753 and 0.729), and there were statistical differences ( P<0.01 or <0.05); the sensitivity of all indexes combined in the differential diagnosis of spinal bone metastasis from lung cancer and myeloma was higher than those of the combination of the CT values at the energy levels of 40 to 80 keV by dual energy CT, the combination of serum PⅠNP and β-CTX levels (98.15% vs. 83.33% and 81.48%), the same as AUC (0.976 vs. 0.882 and 0.829), and there were statistical differences ( P<0.01); there were no significant differences in the specificity of each index alone and combination ( P>0.05). Conclusions:Compared with myeloma, the CT values at the energy levels of 40 to 80 keV by dual energy CT, serum PⅠNP and β-CTX levels in patients with spinal bone metastasis from lung cancer are increased, and the combination of the above indexes has ideal value in differential diagnosis of the two diseases.

The origin and classification of cortical projection neuron / 国际儿科学杂志

As the main cell type in mammalian cortex, cortical projection neurons play an important role in brain functions, including motor control, sensation and cognition.Projection neurons in the cerebral cortex include excitatory projection neurons and inhibitory projection neurons, of which excitatory projection neurons are the main type.Cortical excitatory projection neurons have various subtypes, and most of them have their own unique developmental rules and molecular markers.Studying the origin and classification of projection neurons in the cerebral cortex is helpful to understand the mechanisms of diseases related to abnormal projection neurons.This paper reviews the origin, classification and function of cortical projection neurons.

COPD identification using maximum intensity projection of lung field CT images and deep convolution neural network / 中华健康管理学杂志

Objective:To propose a model using the maximum intensity projection (MIP) of lung field computed tomography (CT) images and deep convolution neural network (CNN) and explore its value in identifying chronic obstructive pulmonary disease (COPD).Methods:A total of 201 subjects were selected from the Second Hospital of Dalian Medical University from January 2010 to May 2021. All subjects were included according to the inclusion criteria and were divided into COPD group (101 cases) and healthy controls group (100 cases). Each patient underwent a high-resolution CT scan of the chest and pulmonary function test. First, the lung field was extracted from CT images and the intrapulmonary MIP images were acquired. Second, with these MIP images as input, the model for identifying COPD was constructed based on a modified residual network (ResNet). Finally, the influence of the number of residual blocks on the performance of the models was investigated. Accuracy, sensitivity, specificity, positive predictive value, negative predictive value, receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to evaluate the identification efficiency.Results:The accuracy, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) of ResNet26 was 76.1%, 76.2%, 76.0%, 76.2%, and 76.0%, respectively; and the AUC of the test was 0.855 (95% CI: 0.799-0.901). The accuracy, sensitivity, specificity, PPV, NPV of ResNet50 was 77.6%, 76.2%, 79.0%, 78.6%, and 76.7%, respectively; and the AUC of the test was 0.854 (95% CI: 0.797-0.900). The accuracy, sensitivity, specificity, PPV, NPV of ResNet26d was 82.1%, 83.2%, 81.0%, 81.6%, and 82.7%, respectively; and the AUC of the test was 0.885 (95% CI: 0.830-0.926). Conclusions:The COPD identification model via MIP images from CT images within the lung and deep CNN is successfully constructed and achieves accurate COPD identification. And it can provide an effective tool for COPD screening.

Application value of artificial intelligence in dual-source CT coronary angiography / 中国基层医药

Objective:To investigate the application value of artificial intelligence in dual-source CT coronary angiography.Methods:The imaging data of 50 patients with coronary artery diseases who received treatment in Benxi Central Hospital from January to December 2021 were retrospectively analyzed. All patients underwent coronary computed tomography angiography examination and coronary arteriography. Coronary computed tomography angiography images were uploaded to the post-processing workstation and post-processed and analyzed by two radiologists. At the same time, the images were also post-processed by AI software and diagnosis reports were generated. In terms of coronary artery stenosis, the diagnostic results provided by AI software were compared with coronary angiography results. In terms of myocardial bridging, the diagnostic results provided by AI software were compared with radiologist's diagnosis. The accuracy of AI software was judged.Results:In the diagnosis of coronary artery stenosis, the sensitivity, specificity, positive predictive value and the negative predictive value of AI software were 93.22%, 81.32%, 76.39% and 94.87% respectively. These were well consistent with coronary arteriography results (Kappa = 0.71, P < 0.001). In the diagnosis of myocardial bridging, the sensitivity, specificity, positive predictive value and the negative predictive value of AI software were 30.77%, 81.45%, 25.81% and 84.87% respectively. These were poorly consistent with radiologists' diagnostic results (Kappa = 0.11, P = 0.162). Conclusion:AI-based dual-source CT coronary angiography is of high value in the diagnosis of coronary artery stenosis, but it is of low value in the diagnosis of myocardial bridging.

Low-dose helical CT projection data restoration using noise estimation / 南方医科大学学报

OBJECTIVE@#To build a helical CT projection data restoration model at random low-dose levels.@*METHODS@#We used a noise estimation module to achieve noise estimation and obtained a low-dose projection noise variance map, which was used to guide projection data recovery by the projection data restoration module. A filtering back-projection algorithm (FBP) was finally used to reconstruct the images. The 3D wavelet group residual dense network (3DWGRDN) was adopted to build the network architecture of the noise estimation and projection data restoration module using asymmetric loss and total variational regularization. For validation of the model, 1/10 and 1/15 of normal dose helical CT images were restored using the proposed model and 3 other restoration models (IRLNet, REDCNN and MWResNet), and the results were visually and quantitatively compared.@*RESULTS@#Quantitative comparisons of the restored images showed that the proposed helical CT projection data restoration model increased the structural similarity index by 5.79% to 17.46% compared with the other restoration algorithms (P < 0.05). The image quality scores of the proposed method rated by clinical radiologists ranged from 7.19% to 17.38%, significantly higher than the other restoration algorithms (P < 0.05).@*CONCLUSION@#The proposed method can effectively suppress noises and reduce artifacts in the projection data at different low-dose levels while preserving the integrity of the edges and fine details of the reconstructed CT images.

An adaptive CT metal artifact reduction algorithm that combines projection interpolation and physical correction / 南方医科大学学报

OBJECTIVE@#To propose an adaptive weighted CT metal artifact reduce algorithm that combines projection interpolation and physical correction.@*METHODS@#A normalized metal projection interpolation algorithm was used to obtain the initial corrected projection data. A metal physical correction model was then introduced to obtain the physically corrected projection data. To verify the effectiveness of the method, we conducted experiments using simulation data and clinical data. For the simulation data, the quantitative indicators PSNR and SSIM were used for evaluation, while for the clinical data, the resultant images were evaluated by imaging experts to compare the artifact-reducing performance of different methods.@*RESULTS@#For the simulation data, the proposed method improved the PSNR value by at least 0.2 dB and resulted in the highest SSIM value among the methods for comparison. The experiment with the clinical data showed that the imaging experts gave the highest scores of 3.616±0.338 (in a 5-point scale) to the images processed using the proposed method, which had significant better artifact-reducing performance than the other methods (P < 0.001).@*CONCLUSION@#The metal artifact reduction algorithm proposed herein can effectively reduce metal artifacts while preserving the tissue structure information and reducing the generation of new artifacts.

Modelo dinámico para proyectar la necesidad de recursos humanos en salud: anestesistas en Uruguay / Anesthesiologists in Uruguay: dynamic model to project the need of human resources in healthcare

Objetivo Desarrollar un modelo dinámico para simular la oferta de médicos especialistas y estimar la brecha especto a la demanda/necesidad en anestesiología en el sistema de salud del Uruguay. Métodos Se desarrolló un modelo de simulación dinámico determinístico implementado en el programa libre R. Se analizaron las proyecciones en el período 2011-2050 y se estimó la brecha a partir de la situación de equilibrio o desequilibrio entre oferta y demanda/necesidad. Se evaluó la calidad del modelo comparando los valores simulados con los datos históricos, con indicadores de bondad de ajuste, como la raíz del error cuadrático medio relativo (rRMSE). Se realizó un análisis de sensibilidad con respecto a los cupos de ingreso a la especialidady la tasa de crecimiento de la necesidad de especialistas. Resultados Se proyectó la oferta y demanda de anestesistas para el período considerado. Se obtuvo un rRMSE menor a 0,1, lo que sugiere que el modelo propuesto reproduce adecuadamente la dinámica de la oferta real. Para el período proyectado la situación a mediano y largo plazo es de equilibrio. Conclusión El modelo simulado presenta buen ajuste, por lo que la proyección de la oferta de Recursos Humanos (RR. HH.) representa de forma precisa la disponibilidad futura de la fuerza de trabajo. Además, el modelo representa un insumo de interés para la gestión informada sobre la necesidad de recursos humanos y las políticas de salud, dado que permite evaluar las proyecciones bajo diferentes escenarios.

Objectives The aim of this study is to develop a dynamic model to simulate the supply of specialized physicians in Anesthesiology and estimate the gap with its demand, within Uruguay healthcare system. Methods A deterministic dynamic simulation model was developed and implemented using R software. Projections for the 2011-2050 period were analyzed, and the gap was estimated based on the equilibrium state of supply and demand. The quality of the model was evaluated comparing the simulated data with historical empirical data using goodness of fit indicators, such as the relative root mean square error (rRMSE). Results The demand and supply of anesthesiologists was projected for the period under analysis. A rRMSE<0,1 was obtained, which suggests the proposed model adequately reproduces the real offer dynamics. Based on the defined gap criteria, in the medium and long-term the situation is in equilibrium state. Conclusions The simulated model presents a good fit so that the human resources (HR) supply projection represents in a precise way the future availability of the work-force. Given that the model allows to evaluate the projection dynamics under different management scenarios, the model also represents an input of the utmost interest for management knowledgeable about human resource demands and healthcare policy.

Comparison of radiographic and tomographic evaluations for measurement of the Canal Flare Index in dogs / [Comparação entre avaliação radiográfica e tomográfica para mensuração do Canal Flare Index em cães]

The outcome of total hip arthroplasty (THA) in dogs is directly related to surgical planning. Templating of radiographs prior to THA should help the surgeon anticipate prosthesis size and femoral shape allowing canal fill of the proximal metaphysis by the implant ensuring primary stable fixation. The canal flare index (CFI) obtained from radiograph has been used as a measure of risk of complications for the technique in human beings and dogs. However, standard radiographs only provide limited data for the selection of cementless prostheses and the assessment of their fit within the femoral canal, due to factors like radiographic magnification and femoral rotation. Therefore, three-dimensional evaluation based on computed tomography (CT) may be a better tool for CFI measurement. The aim of this study was to compare anatomical measurement with CFI values obtained from craniocaudal radiography and CT. Craniocaudal radiographs using a horizontal radiographic beam (CR), CT, and anatomical macroscopic measurements (A) were obtained from 45 femurs from 23 canine cadavers. The differences between the values of CFI obtained from radiograph (CFI-R), computed tomography on transverse (CFI- TT) and longitudinal axis (CFI-TL) compared to the CFI obtained from macroscopic measurements - gold standard - (CFI-A), and 95% limits of agreement (LOA) between the values, were evaluated by the Bland-Altman method. Dimensions obtained from CT techniques had a greatest mean difference from anatomical and CFI values were also different (P=0.032). Under the experimental conditions, the craniocaudal radiograph, provided the most accurate measurement of the CFI (mean difference: 0.087 ± 0.42).(AU)

O resultado da artroplastia total do quadril (ATQ) em cães está diretamente relacionado ao planejamento cirúrgico. O templating radiográfico pré-operatório da ATQ deve ajudar o cirurgião a prever o tamanho da prótese e o formato do fêmur, o que permitirá um preenchimento ideal da metáfise proximal pelo implante, garantindo, assim, fixação primária estável. O índice de alargamento do canal (Canal Flare Index - CFI) obtido em radiografias tem sido utilizado como fator de risco de complicações para a técnica em humanos e cães. No entanto, as radiografias podem fornecer apenas dados limitados para a seleção de próteses não cimentadas e a avaliação do seu encaixe no canal femoral, devido a fatores como ampliação radiográfica e rotação femoral. Portanto, a avaliação tridimensional baseada na tomografia computadorizada (TC) pode ser uma ferramenta vantajosa para a mensuração do CFI. O objetivo deste estudo foi comparar a medida anatômica com os valores de CFI obtidos na radiografia craniocaudal e na TC. Radiografias craniocaudais utilizando feixe radiográfico horizontal (CR), tomografia computadorizada e medidas macroscópicas anatômicas (A) foram obtidas de 45 fêmures de 23 cadáveres caninos. As diferenças entre os valores de CFI obtidos na radiografia (CFI-R), na tomografia computadorizada no eixo transversal (CFI-TT) e no eixo longitudinal (CFI-TL), em comparação com os valores de CFI obtidos nas medições macroscópicas - padrão-ouro - (CFI-A) e os limites de concordância de 95% (LOA) entre os valores, foram avaliadas pelo método de Bland-Altman. As dimensões obtidas pelas técnicas de TC apresentaram maior diferença média dos valores anatômicos, e as do CFI também foram diferentes (P=0,032). Nas condições experimentais, a radiografia craniocaudal forneceu a medida mais precisa do CFI (diferença média: 0,087 ± 0,42) para representar o padrão-ouro deste estudo.(AU)

Medium / 中南大学学报(医学版)

OBJECTIVES@#The rapid growth of health expenditure has always been the focus of health policy. This study aims to project health expenditure in Shanghai and to carry out policy simulations on the impact of chronic disease prevention programs on health costs in the Healthy Shanghai Initiative.@*METHODS@#Based on the Shanghai health accounts, component-based model was used to project Shanghai total health expenditure of 2020-2035, and the policy stimulation was implemented.@*RESULTS@#In 2020-2035, Shanghai's health expenditure is expected to grow continually, the proportion of total health expenditure in GDP will exceed 8.00% in 2023, reach 9.00% in 2025, and 10.03% in 2035. The proportion of current health expenditure in GDP will exceed 8.00% in 2024 and reach 9.55% in 2035. The chronic disease prevention plan help saving the medical expenditure of respiratory diseases,endocrine system diseases, and circulatory system diseases, accounting 3.28% to 10.58% of total health expenditure.@*CONCLUSIONS@#The sustainability of health financing in Shanghai is facing challenges under the new normal of economy. It is necessary to promote the prevention and control of chronic diseases and strengthen cost control from both the supply and demand sides.

Como que as mudanças nas taxas de mortalidade e expectativa de vida afetam a projeção da população idosa no estado de São Paulo? / How do changes in mortality and life expectancy affect the elderly population projection in the state of Sao Paulo, Brazil?

Resumo Introdução O Brasil experimentou, ao longo do século XX, uma profunda mudança em relação à expectativa de vida ao nascer. Nesse processo, foi importante não somente a queda do nível da mortalidade para o grande aumento da expectativa de vida, mas também a mudança no padrão da mortalidade. Recentemente, vêm sendo discutidas mudanças no padrão de mortalidade nas idades mais avançadas, bem como o impacto no envelhecimento populacional. Entretanto, no Brasil, as discussões sobre a longevidade e o comportamento da mortalidade nas idades avançadas ainda são incipientes. Especialistas sugerem que projeções de mortalidade incorporem novas tendências de desaceleração da mortalidade em idades avançadas e explorem abordagens de coortes para suas formulações de tendências. Objetivo Estudar o efeito causado pelas mudanças no comportamento da mortalidade e da expectativa de vida na predição do tamanho da população idosa do estado de São Paulo. Método Método estendido de coortes componentes. Resultados Alterações no nível e no padrão da mortalidade têm um efeito maior na predição da população de 80 anos ou mais em comparação com a população de 60 anos ou mais. Ademais, considerar o gap da expectativa de vida entre os sexos é relevante para determinar o número futuro de idosos. Conclusão Ao utilizar países em diferentes estágios da transição epidemiológica como cenário futuro do padrão e do nível da mortalidade de São Paulo, o método estendido de coortes componentes se torna uma possibilidade metodológica interessante para avaliar o impacto dessas modificações para a projeção da população idosa, podendo ser uma ferramenta para a avaliação de políticas públicas.

Abstract Background Brazil experienced a significant change in life expectancy at birth in the 20th century. In this process, it is important to observe not only the decrease in mortality level and the increase in life expectancy, but also the change in mortality pattern, that is, how mortality occurred and which ages were most impacted. Recently, these changes in the mortality pattern at more advanced ages and their impact on population aging have been discussed more intensively. However, in Brazil, discussions about human longevity and mortality behavior in advanced ages are still incipient. Experts suggest that future studies and mortality projections incorporate new trends in decelerating mortality at advanced ages and explore cohort approaches in their trend formulations. Objective To study the effect of changes in mortality and life expectancy on predicting the elderly population size in the state of Sao Paulo, Brazil. Method Extended cohort-component study. Results Changes in the level and pattern of mortality have a greater effect on the population aged ≥80 compared with that on the population aged ≥60. In addition, considering the life expectancy gap between sexes is relevant to determine the future number of older people. Conclusion The extended cohort component method is an interesting methodological approach to assess the impact of mortality changes on the elderly population projection by using different stages of the epidemiological transition as a future scenario

Divergent Projection Patterns Revealed by Reconstruction of Individual Neurons in Orbitofrontal Cortex / 神经科学通报·英文版

The orbitofrontal cortex (OFC) is involved in diverse brain functions via its extensive projections to multiple target regions. There is a growing understanding of the overall outputs of the OFC at the population level, but reports of the projection patterns of individual OFC neurons across different cortical layers remain rare. Here, by combining neuronal sparse and bright labeling with a whole-brain florescence imaging system (fMOST), we obtained an uninterrupted three-dimensional whole-brain dataset and achieved the full morphological reconstruction of 25 OFC pyramidal neurons. We compared the whole-brain projection targets of these individual OFC neurons in different cortical layers as well as in the same cortical layer. We found cortical layer-dependent projections characterized by divergent patterns for information delivery. Our study not only provides a structural basis for understanding the principles of laminar organizations in the OFC, but also provides clues for future functional and behavioral studies on OFC pyramidal neurons.

La Universidad gestora de procesos de desarrollo local y fortalecimiento de redes: la experiencia de una intervención comunitaria en Cali Colombia / The University as a Manager of Local Development Processes and Network Strengthening: The Experience of Community Intervention in Cali, Colombia

Resumen Se pueden reconocer que estrategias como el "Centro Integral de Promoción de De rechos-Sol de Oriente" de la Universidad Cooperativa de Colombia seccional Cali, la Agencia para la Reincorporación y Normalización (ARN) y la Fundación para la orientación Familiar (FUNOF), posibilitan la promoción de un desarrollo social comunitario que se avoca por promover mejores formas de socialización y vínculos que permitan la construcción de un tejido social promotor de la salud mental. Así mismo, las estrategias de intervención que se orientan a la idea-propuesta de la cultura ciudadana como 'Formula' se han hecho populares y se le adjudican variados logros, entre estos el trabajo por la convivencia en medio de un país profundamente desigual y polarizado, impactado por violencias que se entrelazan.

Abstract Today, it can be recognized that strategies, such as the "Integral Center for the Promotion of Rights" at Universidad Cooperativa de Colombia, Cali campus -, the Agency for Reinstatement and Standardization and Family Guidance Foundation (FUNOF), enable the promotion of a community social development, whose aim is to promote better forms of socialization and links that allow the construction of a social fabric, by promoting mental health. Likewise, intervention strategies, which focus on the idea-proposal of citizen culture as a 'Formula' have become popular, and they have been awarded various achievements, by including work for coexistence, in the midst of a deeply unequal and polarized country, impacted by intertwining violence.

Tocando a vida com os dedos: reciprocidade na clínica de adolescentes / Touching life with our fingers: reciprocity in the clinic with adolescents / Tocando la vida con los dedos: reciprocidad en la clínica de adolescentes / Toucher la vie avec les doigts: réciprocité dans la clinique des adolescents

No artigo, a autora trata a reciprocidade como a reverberação entre analista e analisando, com foco no trabalho psicanalítico com adolescentes. Entende que é na adolescência que as confusões se ativam mais intensamente. A necessidade de destituir a autoridade dos pais externos confunde-se com a queda dos pais internos, o que deflagra a necessidade de identificação introjetiva -processo que, por sua vez, aparece no apego a figuras sociais, grupos musicais, esportivos e culturais. As ideias aqui desenvolvidas partem do conceito de reciprocidade estética (Meltzer, 1992; 1995), culminando em ampliações sobre os processos de identificação e introjeção, que se desenrolam exatamente nessa troca recíproca.

In this article, the author treats reciprocity as the reverberation between analyst and patient, focusing on psychoanalytic work with adolescents. She understands that it is in adolescence that confusions are most intensely activated. The need to remove the authority of external parents is confused with the fall of internal parents, which triggers the need for introjective identification - a process that, in turn, appears in the attachment to social figures, such as musicians, sportspeople and cultural groups. The ideas developed here are based on the concept of aesthetic reciprocity (Meltzer, 1992; 1995), culminating in expansions on the processes of identification and introjection, which take place exactly in this reciprocal exchange.

En el presente artículo, la autora trata la reciprocidad como la reverberación entre analista y analizando, centrándose en el trabajo psicoanalítico con adolescentes. Ella entiende que es en la adolescencia que las confusiones se activan más intensamente. La necesidad de eliminar la autoridad de los padres externos se confunde con la caída de los padres internos, lo que desencadena la necesidad de una identificación introyectiva, un proceso que, a su vez, aparece en el apego a figuras sociales, grupos musicales, deportivos y culturales. Las ideas desarrolladas aquí parten del concepto de reciprocidad estética (Meltzer, 1992; 1995), que culmina en expansiones en los procesos de identificación e introyección, que tienen lugar exactamente en este intercambio recíproco.

Dans cet article, l'auteur traite la réciprocité comme la réverbération entre l'analyste et l'analysant, en se concentrant sur le travail psychanalytique avec les adolescents. Elle comprend que c'est à l'adolescence que les confusions sont le plus intensément activées. La nécessité de retirer l'autorité des parents externes est confondue avec la chute des parents internes, ce qui déclenche la nécessité d'une identification introjective - un processus qui, à son tour, apparaît comme attaché aux figures sociales, aux groupes musicaux, sportifs et culturels. Les idées développées ici partent du concept de réciprocité esthétique (Meltzer, 1992; 1995), aboutissant à des développements sur les processus d'identification et d'introjection, qui se déroulent exactement dans cet échange réciproque.

Facial Angle Profiles in Turkish Healthy Population

The knowledge of the facial angles is essential for safe and accurate plastic surgery interventions. Facial angles are used as a reference value for comparison during diagnosis and treatment planning. Also, the facial angles will provide valuable data in evaluation of facial aesthetics. So, the aim of this study was to determine the ideal values of the facial angles in Turkish healthy adults.METHODSTwo hundred and forty seven (129 females; 118 males) subjects aged between 18 and 25 years were included in this study. Frankfort horizontal plane was chosen to determine the angles related with face. Photographs were acquired using a Digital SLR Camera with fixed shooting values. (Canon EOS 80D; ISO 100 f/4.5). In all the shoots, a printed scale with known dimensions was present. Acquired images were then transferred to a computer station. Measurements were made using Image J 1.52a with 1/100 mm sensitivity. Statistical analysis was done using SPSS Ver. 22.00. A p<0.05 value was considered as significant. Student’s T Test was used to determine the significance between gender, while Pearson Correlation analysis was done to evaluate the relation between gender.RESULTSStatistically significant difference was found between the genders and the frontonasal angle, the nasofrontal angle, the nasolabial angle, the chin neck angle, and the nasal projection. Also, the fronto nasal angle, chin neck angle, and nasal projection measurements were higher in males than in females, whereas the nasofrontal angle and nasolabial angle were lower in males than in females. Additionally, there was a significant, negative very low correlation between fronto-nasal angle (-0.148), chin-neck angle (r=-0.179) and gender; a significant, negative and low correlation (r=-0.243) between nasal projection and gender, a significant, positive and low correlation between nasolabial angle and gender (r=0.259); and nasofrontal angle and gender (r=0.388).CONCLUSIONSFacial angle values of healthy population provide important and useful knowledge in terms of comparison of abnormalities clinically, and data may be valuable for the representatives of clinical disciplines.

Estimation and prediction of adult AIDS epidemic in Wuhan using EPP/Spectrum model / 公共卫生与预防医学

Objective To estimate and predict the characteristics and development trend of the AIDS epidemic situation in Wuhan by using Estimation and Projection Package (EPP) and Spectrum model. Methods Data from HIV/AIDS case reports, sentinel surveillance, special investigations, maternal screenings, detention places reports, antiviral treatments, and screenings of high-risk populations was collected, verified and sorted out. Estimation and projection package (EPP) and Spectrum models were used to fit and modify the curves. The final results were formed after consultation and discussion with experts. Results The EPP/Spectrum model predicted that the HIV infection rate of adults in Wuhan increased significantly after 2003, reached the peak around 2013, and then maintained a high level until 2019. The HIV infection rate of adults in 2019 was about 9.0/105, and the adult infection rate would show a downward trend after 2020. Among all kinds of populations, the infection rate of spouses/sexual partners of already infected people was the highest, with the infection rate in men who have sex with men (MSM) ranking second. The number of people living with HIV/AIDS increased gradually from 2000, and by 2022 the number is estimated to be around 8,000 (95% CI:5421-9754). In regards to gender and age, the main distribution of HIV infections was among young adults, especially men aged 25-50 years old. Conclusion Wuhan currently is a low prevalence area of AIDS. MSM will still be the focus of intervention in the following years.

In Vivo Two-photon Calcium Imaging in Dendrites of Rabies Virus-labeled V1 Corticothalamic Neurons / 神经科学通报·英文版

Monitoring neuronal activity in vivo is critical to understanding the physiological or pathological functions of the brain. Two-photon Ca imaging in vivo using a cranial window and specific neuronal labeling enables real-time, in situ, and long-term imaging of the living brain. Here, we constructed a recombinant rabies virus containing the Ca indicator GCaMP6s along with the fluorescent protein DsRed2 as a baseline reference to ensure GCaMP6s signal reliability. This functional tracer was applied to retrogradely label specific V1-thalamus circuits and detect spontaneous Ca activity in the dendrites of V1 corticothalamic neurons by in vivo two-photon Ca imaging. Notably, we were able to record single-spine spontaneous Ca activity in specific circuits. Distinct spontaneous Ca dynamics in dendrites of V1 corticothalamic neurons were found for different V1-thalamus circuits. Our method can be applied to monitor Ca dynamics in specific input circuits in vivo, and contribute to functional studies of defined neural circuits and the dissection of functional circuit connections.